High reproducibility measurements for pupil size in both dim and bright light conditions were found, with Cronbach’s alpha values of 0.987 and
0.985, respectively.
Intraclass Correlation Coefficient (ICC) for average measures was 0.981 (95% CI: 0.959 to 0.991) for dim light and 0.973 (95% CI: 0.909 to 0.988)
for bright light.
For accommodation measurements, the results demonstrated good consistency, with a Cronbach’s alpha of 0.837 and an ICC for average
measures of 0.829 (95% CI: 0.724 to 0.898).
Pupillary reactivity was categorized into three groups based on constriction velocity: non-reactive (velocity < 0.4 mm/s), sluggish (0.4 mm/s ≤ velocity < 0.8 mm/s), and brisk (velocity ≥ 0.8 mm/s). The measured reproducibility among these categories was 100%.
Table 1. Reproducibility analysis of pupillary metrics in control subjects
| Metric | Cronbach’s alpha | ICC – Average Measures | 95% CI (Avg) | ICC – Single Measures | 95% CI (Single) |
|---|---|---|---|---|---|
| Dim Light (Diameter, mm) | 0.987 | 0.981 | 0.959–0.991 | 0.946 | 0.886–0.973 |
| Bright Light (Diameter, mm) | 0.985 | 0.973 | 0.909–0.988 | 0.922 | 0.770–0.966 |
| Reactive Velocity (mm/s) | 0.716 | 0.717 | 0.544–0.832 | 0.458 | 0.284–0.622 |
| Pupil Reactivity Category | 1.000 | 1.000 | — | 1.000 | — |
| Accommodation (mm) | 0.837 | 0.829 | 0.724–0.898 | 0.618 | 0.467–0.747 |
ICC = Intraclass Correlation Coefficient; CI = Confidence Interval